immunofluorescence using a mouse α-FLAG antibody followed by Alexa-coupled secondary IgG.b, cells were incubated with 30 nmTeNT for an additional 48 h.VAMP2cleavage was visualized byWestern blotting, using a mouse α-Syb2 antibody, which recognizes only full-length VAMP2.BSA, bovine serum ...
Previously, we reported that GD3+ cells show stronger tyrosine phosphorylation of focal adhesion kinase (FAK), p130Cas, and paxillin when treated with fetal calf serum than GD3− cells. In this study, we analyzed the changes in the signals mediated by the interaction between integrins and ...
Cells were grown in growth medium (RPMI 1640 medium containing 10% fetal bovine serum) at 37 °C under a humidified 5% CO2 atmosphere. For EMT induction, cells were cultured for 48 h in growth medium containing 10 ng/mL TGF-β1 (Peprotech, Rocky Hill, NJ, USA). For 3D ...
Sera from four chimpanzees and two monkeys were evaluated for anti-GD3 antibody activity by solid-phase radioimmunoassay using GD3 and control gangliosides as targets. Serum from one monkey, immunized with cells from a melanoma cell line, was strongly reactive with GD3, having a titer of >2500....
BSA, bovine serum albumin TBS, Tris-buffered saline QCM, quartz-crystal microbalance TU, transducing units CTB, cholera toxin B subunit 1. Introduction Carbohydrate-protein interaction is an important process in cell-cell recognition [1]. Thus, it is expected that the peptides which inhibit carboh...
A preparation of c-series ganglioside-specific monoclonal antibody A2B5 was obtained as follows. A2B5-producing hybridomas (CRL 1520, American Type Culture Collection, USA) were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum. The culture medium containing the ...
A panel of test samples containing varying amounts of anti-G M1 antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound G M1 ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ...
At the end of the 12-week treatments of nanoparticles, the mice were fasted for 8 h, and the blood of each mouse was collected for serum preparation. The concentrations of four major lipids in sera including total cholesterol (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL...